Establishment and functional characterization of a tracheal epithelial cell line RTEC11 from transgenic rats harboring temperature‐sensitive simian virus 40 large T‐antigen
Identifieur interne : 000800 ( Istex/Checkpoint ); précédent : 000799; suivant : 000801Establishment and functional characterization of a tracheal epithelial cell line RTEC11 from transgenic rats harboring temperature‐sensitive simian virus 40 large T‐antigen
Auteurs : Yoshiaki Tabuchi [Japon] ; Takeshi Doi [Japon] ; Ichiro Takasaki [Japon] ; Ri-Ichi Takahashi [Japon] ; Masatsugu Ueda [Japon] ; Yoshihisa Suzuki [Japon] ; Masuo Obinata [Japon]Source :
- Cell Biology International [ 1065-6995 ] ; 2008-11.
Abstract
A tracheal epithelial cell line RTEC11 was established from transgenic rats harboring temperature‐sensitive simian virus 40 large T‐antigen. The cells grew continuously at a permissive temperature of 33 °C but not at a non‐permissive temperature of 39 °C. Morphological and functional investigations demonstrated that the cells were polarized epithelial cells maintaining a regulated permeability barrier function. Interestingly, the expression levels of Muc1 (mucin 1) and Scgb1a1 (uteroglobin), non‐ciliated secretory cell markers, and Tubb4 (tubulin beta 4), a ciliated cell marker, were significantly increased under the cell growth‐restricted condition. Global gene expression and computational network analyses demonstrated a significant genetic network associated with cellular development and differentiation in cells cultured at the non‐permissive temperature. The tracheal epithelial cell line RTEC11 with unique characteristics should be useful as an in vitro model for studies of the physiological functions and gene expression of tracheal epithelial cells.
Url:
DOI: 10.1016/j.cellbi.2008.07.025
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:C578B217F6E4D5CB6A22A4A4B9E660766A88A23FLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Establishment and functional characterization of a tracheal epithelial cell line RTEC11 from transgenic rats harboring temperature‐sensitive simian virus 40 large T‐antigen</title><author><name sortKey="Tabuchi, Yoshiaki" sort="Tabuchi, Yoshiaki" uniqKey="Tabuchi Y" first="Yoshiaki" last="Tabuchi">Yoshiaki Tabuchi</name></author><author><name sortKey="Doi, Takeshi" sort="Doi, Takeshi" uniqKey="Doi T" first="Takeshi" last="Doi">Takeshi Doi</name></author><author><name sortKey="Takasaki, Ichiro" sort="Takasaki, Ichiro" uniqKey="Takasaki I" first="Ichiro" last="Takasaki">Ichiro Takasaki</name></author><author><name sortKey="Takahashi, Ri Chi" sort="Takahashi, Ri Chi" uniqKey="Takahashi R" first="Ri-Ichi" last="Takahashi">Ri-Ichi Takahashi</name></author><author><name sortKey="Ueda, Masatsugu" sort="Ueda, Masatsugu" uniqKey="Ueda M" first="Masatsugu" last="Ueda">Masatsugu Ueda</name></author><author><name sortKey="Suzuki, Yoshihisa" sort="Suzuki, Yoshihisa" uniqKey="Suzuki Y" first="Yoshihisa" last="Suzuki">Yoshihisa Suzuki</name></author><author><name sortKey="Obinata, Masuo" sort="Obinata, Masuo" uniqKey="Obinata M" first="Masuo" last="Obinata">Masuo Obinata</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C578B217F6E4D5CB6A22A4A4B9E660766A88A23F</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1016/j.cellbi.2008.07.025</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-79QW1W1D-F/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001339</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001339</idno><idno type="wicri:Area/Istex/Curation">001339</idno><idno type="wicri:Area/Istex/Checkpoint">000800</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000800</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Establishment and functional characterization of a tracheal epithelial cell line RTEC11 from transgenic rats harboring temperature‐sensitive simian virus 40 large T‐antigen</title><author><name sortKey="Tabuchi, Yoshiaki" sort="Tabuchi, Yoshiaki" uniqKey="Tabuchi Y" first="Yoshiaki" last="Tabuchi">Yoshiaki Tabuchi</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, 2630 Sugitani, 930‐0194 Toyama</wicri:regionArea><wicri:noRegion>930‐0194 Toyama</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Japon</country></affiliation></author><author><name sortKey="Doi, Takeshi" sort="Doi, Takeshi" uniqKey="Doi T" first="Takeshi" last="Doi">Takeshi Doi</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, 2630 Sugitani, 930‐0194 Toyama</wicri:regionArea><wicri:noRegion>930‐0194 Toyama</wicri:noRegion></affiliation></author><author><name sortKey="Takasaki, Ichiro" sort="Takasaki, Ichiro" uniqKey="Takasaki I" first="Ichiro" last="Takasaki">Ichiro Takasaki</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Molecular Genetics Research, Life Science Research Center, University of Toyama, 2630 Sugitani, 930‐0194 Toyama</wicri:regionArea><wicri:noRegion>930‐0194 Toyama</wicri:noRegion></affiliation></author><author><name sortKey="Takahashi, Ri Chi" sort="Takahashi, Ri Chi" uniqKey="Takahashi R" first="Ri-Ichi" last="Takahashi">Ri-Ichi Takahashi</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>The YS Institute, Inc., Utsunomiya, Tochigi</wicri:regionArea><wicri:noRegion>Tochigi</wicri:noRegion></affiliation></author><author><name sortKey="Ueda, Masatsugu" sort="Ueda, Masatsugu" uniqKey="Ueda M" first="Masatsugu" last="Ueda">Masatsugu Ueda</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>The YS Institute, Inc., Utsunomiya, Tochigi</wicri:regionArea><wicri:noRegion>Tochigi</wicri:noRegion></affiliation></author><author><name sortKey="Suzuki, Yoshihisa" sort="Suzuki, Yoshihisa" uniqKey="Suzuki Y" first="Yoshihisa" last="Suzuki">Yoshihisa Suzuki</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Obinata, Masuo" sort="Obinata, Masuo" uniqKey="Obinata M" first="Masuo" last="Obinata">Masuo Obinata</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Cell Biology International</title><title level="j" type="alt">CELL BIOLOGY INTERNATIONAL</title><idno type="ISSN">1065-6995</idno><idno type="eISSN">1095-8355</idno><imprint><biblScope unit="vol">32</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1344">1344</biblScope><biblScope unit="page" to="1352">1352</biblScope><biblScope unit="page-count">9</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2008-11">2008-11</date></imprint><idno type="ISSN">1065-6995</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1065-6995</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A tracheal epithelial cell line RTEC11 was established from transgenic rats harboring temperature‐sensitive simian virus 40 large T‐antigen. The cells grew continuously at a permissive temperature of 33 °C but not at a non‐permissive temperature of 39 °C. Morphological and functional investigations demonstrated that the cells were polarized epithelial cells maintaining a regulated permeability barrier function. Interestingly, the expression levels of Muc1 (mucin 1) and Scgb1a1 (uteroglobin), non‐ciliated secretory cell markers, and Tubb4 (tubulin beta 4), a ciliated cell marker, were significantly increased under the cell growth‐restricted condition. Global gene expression and computational network analyses demonstrated a significant genetic network associated with cellular development and differentiation in cells cultured at the non‐permissive temperature. The tracheal epithelial cell line RTEC11 with unique characteristics should be useful as an in vitro model for studies of the physiological functions and gene expression of tracheal epithelial cells.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Tōhoku</li></region><settlement><li>Sendai</li></settlement><orgName><li>Université du Tōhoku</li></orgName></list><tree><country name="Japon"><noRegion><name sortKey="Tabuchi, Yoshiaki" sort="Tabuchi, Yoshiaki" uniqKey="Tabuchi Y" first="Yoshiaki" last="Tabuchi">Yoshiaki Tabuchi</name></noRegion><name sortKey="Doi, Takeshi" sort="Doi, Takeshi" uniqKey="Doi T" first="Takeshi" last="Doi">Takeshi Doi</name><name sortKey="Obinata, Masuo" sort="Obinata, Masuo" uniqKey="Obinata M" first="Masuo" last="Obinata">Masuo Obinata</name><name sortKey="Suzuki, Yoshihisa" sort="Suzuki, Yoshihisa" uniqKey="Suzuki Y" first="Yoshihisa" last="Suzuki">Yoshihisa Suzuki</name><name sortKey="Tabuchi, Yoshiaki" sort="Tabuchi, Yoshiaki" uniqKey="Tabuchi Y" first="Yoshiaki" last="Tabuchi">Yoshiaki Tabuchi</name><name sortKey="Takahashi, Ri Chi" sort="Takahashi, Ri Chi" uniqKey="Takahashi R" first="Ri-Ichi" last="Takahashi">Ri-Ichi Takahashi</name><name sortKey="Takasaki, Ichiro" sort="Takasaki, Ichiro" uniqKey="Takasaki I" first="Ichiro" last="Takasaki">Ichiro Takasaki</name><name sortKey="Ueda, Masatsugu" sort="Ueda, Masatsugu" uniqKey="Ueda M" first="Masatsugu" last="Ueda">Masatsugu Ueda</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000800 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 000800 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Checkpoint
|type= RBID
|clé= ISTEX:C578B217F6E4D5CB6A22A4A4B9E660766A88A23F
|texte= Establishment and functional characterization of a tracheal epithelial cell line RTEC11 from transgenic rats harboring temperature‐sensitive simian virus 40 large T‐antigen
}}
| This area was generated with Dilib version V0.6.33. |  |